Cas9 function and host genome sampling in Type II-A CRISPR–Cas adaptation

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Cas9 function and host genome sampling in Type II-A CRISPR-Cas adaptation.

To acquire the ability to recognize and destroy virus and plasmid invaders, prokaryotic CRISPR-Cas systems capture fragments of DNA within the host CRISPR locus. Our results indicate that the process of adaptation by a Type II-A CRISPR-Cas system in Streptococcus thermophilus requires Cas1, Cas2, and Csn2. Surprisingly, we found that Cas9, previously identified as the nuclease responsible for u...

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Type II CRISPR/Cas9 approach in the oncological therapy

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ژورنال

عنوان ژورنال: Genes & Development

سال: 2015

ISSN: 0890-9369,1549-5477

DOI: 10.1101/gad.257550.114